Didekantasi and cell-free supernatant yangdiperoleh used in fermentation using 800 ml of complex medium optimal (with optimal pH and glucose concentration) at a temperature of 30 oC. Sebanyak105 mL of fermentation fluid were sampled every 6-12 hours. Disentrifuga samples for 30 min at 6000 rpm and the supernatant didekantasi. 1 mL of supernatant samples used for analysis of glucose levels with Smogy-Nelson method and 100 mL of distilled and analyzed levels of ethanol. 2.7. Fermentation with cells immobilized to Obtain Glucose Consumption and Production of Ethanol 80 ml of liquid stock biomass disentrifuga at 6000 rpm for 30 minutes. Didekantasi and cell-free supernatant obtained was immobilized in order to stem 2% a manner previously described. All subsequent immobilized cells used in fermentation using 800 ml of complex media in optimum condition and temperature of 30 oC.Cairan fermentation of 105 mL was taken and Son Elevri Ethanol Production Using Saccharomyces cerevisiae immobilized In order to stem 108 ©
Chemistry ITS - IPR Javaas sampled every 6-12 hours. Disentrifuga samples for 30 min at 6000 rpm and the supernatant didekantasi. 1 mL of supernatant samples used for analysis of glucose levels with Smogy-Nelson method and 100 mL of distilled and analyzed levels of ethanol. 2.8. To determine the influence of the test loop iteration use of immobilized cells on levels of ethanol produced, immobilized cells (stem to 2%) is used repeatedly in the fermentation using complex media in optimum condition and temperature of 30 ° C for optimum time (as seen from the curve of ethanol as a function of time that has previously acquired). After the first fermentation is complete, immobilized cells were separated by filtered and directly incorporated into a new fermentation medium for the subsequent fermentation with the same condition. This treatment was repeated five times and analyzed levels of ethanol produced at each iteration of fermentation.
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